Cov khoom siv no yog siv rau hauv vitro qualitative detecting ORF1ab/N/S genes of novel coronavirus 2019-nCoV in respiratory specimens including oropharyngeal swabs, nasopharyngeal swab, sputum and performing mutation typing on HV69- 70 del ib txhij.Raws li tau tshaj tawm hauv GISAID database, ntau dua 95% Omicron variant sequences qhia suav nrog kev tshem tawm hauv HV69-70, uas tuaj yeem ua rau S gene phiaj tsis ua haujlwm (SGTF) hauv PCR kev soj ntsuam.SGTF tuaj yeem siv los ua tus cim npe los tshuaj xyuas Omicron.
Primer sets and FAM labeled probe is designed for specific detection of ORF lab gene of 2019-nCoV, VIC labeled probe for N gene of 2019-nCoV, ROX labeled probe for S gene HV69-70 del mutation of 2019-nCoV.Tib neeg RNase P noob tau muab rho tawm ib txhij nrog cov qauv sim muab kev tswj hwm sab hauv kom siv tau cov txheej txheem nucleic extraction thiab reagent kev ncaj ncees.Kev sojntsuam tsom rau tib neeg RNase P noob tau sau nrog CY5.
Real-Time PCR System: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P, MA 6000;