| 64t ua | 96t ua | ||
| Cheebtsam | Kev noj tshuaj | Cheebtsam | Kev noj tshuaj |
| Reagent phaj | 4 | Lysis buffer B | 2 |
| Lysis buffer B | 1 | Lysis phaj | 1 |
| Lub tes tsho yas | 8 | Ntxuav 1 phaj | 1 |
| Tus txheej txheem phau ntawv | 1 | Ntxuav 2 phaj | 1 |
|
|
| Elution phaj | 1 |
|
|
| Lub tes tsho yas | 1 |
|
|
| Tus txheej txheem phau ntawv | 1 |
96-zoo Round Hole Phaj Npaj
64T Cheebtsam rau coj zoo phaj raws li hauv qab no:
| Nyob zoo-site | 10r7 | 2or8 | 3o9 ib | 4or10 | 5orll | 6 ua 2 |
| Kit Cheebtsam | Lysis Tsis pub 600 mL | Ntxuav Tsis pub 1 500 mL | Ntxuav Tsis pub 2 500 mL | Tsis muaj | Sib nqus Cov hlaws dai 310l ua | Elute Tsis pub l00μL |
Fsau 64t:.
Ua tib zoo tshem lub tshav kub sealing zaj duab xis ntawm lub phaj reagent, thiab tom qab ntawd ntxiv 200μL ntawm cov qauv thiab 20μL ntawm lysis tsis Buffer B rau hauv 1/7 kem ntawm reagent phaj.
Rau cov khoom siv 96T:
Ua tib zoo tshem tawm cov cua sov sealing zaj duab xis ntawm lub phaj reagent, thiab tom qab ntawd ntxiv 200μL ntawm cov qauv thiab 20μL ntawm lysis tsis B rau hauv lysis phaj.
Ntxig lub phaj reagent thiab lub tes tsho yas rau hauv qhov chaw ntawm lub ntsuas ntsuas hauv kev txiav txim, thiab tom qab ntawd nyem rau kev khiav "DNA / RNA" kev pabcuam extraction ntawm nucleic acid extractor.
Thaum kawg ntawm qhov kev pab cuam, tshem lub tes tsho yas thiab muab pov tseg.
Tshem tawm cov phaj elution, thiab cov eluent tau muab rho tawm thiab khaws cia rau hauv lub raj centrifuge tshiab rau kev sim hauv qab.Yog tias qhov kev sim qis qis tsis tuaj yeem ua tiav raws sijhawm, cov qauv DNA tuaj yeem khaws cia ntawm -20 ℃ thiab cov qauv RNA tuaj yeem khaws cia ntawm -80 ℃.